Specialized chemosensory structures

Arthropods

Arthropods (e.g., crabs, insects, spiders) are unique among invertebrates in that they have clearly separate senses of taste and olfaction that are comparable to those of vertebrates. Similar to nematodes, arthropods have a continuous layer of cuticle covering the outside of the body that separates the epidermis from the environment. For chemoreception to occur, the chemosensory cells must be exposed to the environment, and this is achieved through small pores in the cuticle. Most commonly the pores are in hairlike extensions of the cuticle that enclose the outer ends (dendrites) of the receptor cells. Two basic types of structure are recognized: those with a single pore, about 0.15–2 μm in diameter, at the tip of the hair (uniporous) and those with many small pores, about 10 nm in diameter, scattered over the surface of the hair (multiporous). These types are associated with the senses of taste and smell, respectively.

Taste receptor sensilla of arthropods occur mainly on feeding appendages associated with but located outside the mouth. They often occur in groups. In addition, many arthropods have taste receptors on the legs, especially on the ventral surfaces of the tarsi (feet), where they come into contact with whatever the animal is walking on. In some species similar receptors are scattered over the surface of the body and may also be present on egg-laying apparatus.

It is common for four taste receptor cells to be associated with each hair; however, unlike the taste receptor cells of vertebrates, these cells have axons that extend directly, without any synapses, to the central nervous system. Arthropods are segmented animals and have a nerve ganglion in each segment, although the ganglia often become fused together. The axons of taste receptor cells extend only as far as the ganglion of the segment on which they occur, and there is no “taste centre,” to which all information concerning taste is conveyed, in the central nervous system.

The taste receptors of insects, which are the most studied of the arthropods, respond mainly to food-related chemicals, and the sensitivities of the cells vary depending on the nature of the insect’s food. In most plant-feeding species the four cells within a hair may respond most actively to sugars, amino acids, inorganic salts, and a range of compounds produced by plants that generally inhibit feeding. These four categories roughly correspond to the human sweet, sour, salt, and bitter modalities. Bloodsucking insects have receptor cells that are sensitive to adenine nucleotides (adenosine diphosphate [ADP] and adenosine triphosphate [ATP]), and some insects, such as mosquitoes and blowflies, have cells that respond to very low salt concentrations. Apart from bitter-sensitive cells, these cells usually respond to only limited ranges of compounds, even within the class of chemicals to which they are sensitive. For example, a cell may respond to glucose and sucrose but not to fructose, and amino acid-sensitive cells respond to only some amino acids. However, different cells may be sensitive to different groups of these compounds, providing many insects with the capacity to distinguish between suites of amino acids or, sometimes, different sugars. This presumably reflects the occurrence of different receptor proteins in the cell membranes, but little is known about this in insects. In the black blowfly there is evidence that the receptor cells responding to sugars have two receptor proteins, one that recognizes glucose and sucrose and another that recognizes fructose. Since both types of sugar stimulate receptors on the same cell, the fly is unable to distinguish them; a similar arrangement probably occurs in humans. If the receptor proteins were on different cells, the insect would be able to distinguish between the two types of sugar, and this is the case in some insect species.

Some plant-feeding insects that feed on only one or a few closely related plant species have taste receptor cells specialized to perceive chemicals specific to the host. For example, plants in the cabbage family (crucifers) are characterized by a class of compounds called glucosinolates, and some crucifer-feeding insects have cells that respond only to glucosinolates, often exhibiting greatest sensitivity to the specific glucosinolates that occur in their normal hosts. Adult butterflies and adults of some plant-feeding flies may have similar receptor cells on their tarsi, facilitating the recognition of host plants on which to lay eggs. Thus, this response is not concerned with indicating the nutritional status of a plant; rather, it provides the insect with a stimulus indicating that the plant is taxonomically appropriate. Some insects also have receptor cells in their taste hairs that recognize pheromones on the surface of other members of the species. Because perception of these chemicals may have nothing to do with feeding (in relation to insects), this type of perception is usually referred to as contact chemoreception rather than taste.

Insects can perceive chemicals on dry surfaces. In this respect, their sense of taste differs from that of vertebrates, which generally perceive compounds in solution. Chemicals on the surface of another insect or on the surface of a leaf are not in solution and are probably conveyed from the insect or leaf surface by carrier proteins in the material covering the nerve endings at the pore.

Olfactory receptors in arthropods are largely restricted to feelerlike structures at the front end of the animal. In crustaceans most multiporous hairs are on the antennules, and in insects they are on the antennae. However, in arachnids multiporous hairs occur in different positions in different groups. The olfactory receptors of scorpions are found in structures called pectines that project from the ventral surface of the second segment of the opisthosoma, and in sunspiders they are found in small flaps of cuticle called malleoli that hang beneath the basal segments of the legs. However, whip spiders and whip scorpions have the first pair of walking legs modified to form antenna-like structures that are extended in front as them as they move. Multiporous hairs are present on these antenniform legs. Some spiders are known to have a sense of smell, but the receptors have not been identified.

The number of multiporous hairs is usually large, since the greater the number, the greater the chance that molecules in low concentrations in the air or water will make contact with a sensillum. In insects the length or complexity of the antennae is a reflection of the numbers of multiporous sensilla. In insects requiring increased sensitivity, the antennae are branched, providing a larger surface area on which more sensilla can be accommodated. The featherlike (plumose) antennae of some male moths, compared with the slender antennae of females of the same species, provide a high degree of surface area and thus a high degree of sensitivity. For example, in the polyphemus moth a male with plumose antennae has over 60,000 multiporous sensilla on one antenna, whereas a female with slender antennae has only about 13,000 sensilla on a single antenna. Each of the multiporous hairs contains the dendrites of two or more olfactory receptor cells, and the total number of receptor cells may be very large. An adult male cockroach can have as many as 195,000 olfactory receptor cells on one antenna, and an adult male tobacco hornworm moth may have from 100,000 to more than 300,000 receptor cells on one antenna. Some crabs have similar numbers of olfactory receptor cells on their antennules.

The axons from the olfactory receptor cells run to the central nervous system, where the axons from all the cells with similar sensory properties converge to a single glomerulus, similar to vertebrates. The position of the clusters of glomeruli forming the olfactory lobe varies in the different groups of arthropods according to the body segment on which the multiporous receptors occur. In insects and crustaceans the glomeruli clusters are in the brain, but in arachnids the clusters occur in more-posterior parts of the central nervous system. In addition, the number of glomeruli varies between species. A mosquito has about 10 glomeruli on each side of its brain, whereas a grasshopper has about 1,000 glomeruli in total. A male cockroach has about 125 glomeruli, and a male tobacco hornworm moth has about 60 glomeruli. On average, about 1,500 axons from olfactory receptor neurons converge on each glomerulus in the cockroach, and about 5,000 axons converge on each glomerulus in the moth. These average convergences are high, but much lower than in vertebrates (25,000 axons per glomerulus), although some individual glomeruli in insects may connect with many more axons. For example, in the male tobacco hornworm moth, about 60,000 olfactory receptor cells respond to one component of the female pheromone. The axons of all these cells converge on one large glomerulus, called a macroglomerular complex, resulting in roughly 60,000 axons connecting to a single glomerulus.

Each olfactory receptor cell in arthropods seems to express only one type of receptor protein, similar to vertebrates. As a result, each cell responds to a specific chemical. This is best illustrated by cells that respond to sex pheromones, in which a difference in the position of a double bond between two carbon atoms can be distinguished.

Many arthropods are able to respond to and differentiate between a wide range of chemical compounds, including pheromones and food-related odours. Many terrestrial species can perceive a range of common compounds with six or seven carbon atoms that are produced by all green plants as metabolic by-products. Bloodsucking insects and some plant-feeders have cells that respond to carbon dioxide, which in blood feeders can provide an important cue to the presence of a host. The characteristic odours of many plants can be perceived and, depending on the insect species, may cause an insect to be attracted to or repelled by the plant. Arthropods also perceive a wide range of odours that have no obvious direct relevance to their lives. This ability is probably necessary for developing learned associations between odours and important but unpredictable factors in the animals’ lives.

Vertebrates

Terrestrial vertebrates

In terrestrial vertebrates the taste receptor system is generally confined to the oral cavity. However, tadpoles, being aquatic, retain the external solitary chemosensory cells found in fish, whereas adult amphibians lack these cells. This indicates that the chemoreceptor system of amphibians reflects their evolutionary position as terrestrial animals that are still dependent on an aquatic environment for breeding. The olfactory system is directly associated with the intake of air during breathing and thus is almost continuously exposed to environmental odours. In addition, most terrestrial vertebrates have a third group of chemoreceptors that form the vomeronasal organ (Jacobson organ). This is a bony or cartilaginous capsule in the nasal cavity, one on each side of the nasal septum. The lumen of the capsule opens through a duct into the nasal cavity or, in some animals, connects with the oral cavity through an opening in the palate. The capsule is filled with fluid and is lined on one side by ciliated receptor cells. The axons from these cells extend to glomeruli, which are separated from those of the primary olfactory system, forming an accessory olfactory bulb. In contrast to the olfactory system, axons from one type of receptor cell project to different glomeruli, and each glomerulus receives input from several types of receptor. In some salamanders and rats the vomeronasal organs are larger in males than in females.

Two families of genes are concerned with producing receptor proteins in the vomeronasal system. These gene families are different from the primary olfactory gene family. In the mouse there are only 200–300 genes associated with producing vomeronasal receptor proteins. As with other vertebrate chemical receptor proteins whose structures are known, the receptor proteins of the vomeronasal system have seven transmembrane domains. Unlike the receptor cells of the taste and olfactory systems, vomeronasal receptor cells adapt slowly, or sometimes not at all, when continuously stimulated; therefore, the transfer of information to the brain is maintained.

In contrast to the primary olfactory system, in which molecules are conveyed to the receptors as an inevitable consequence of breathing, transfer of stimulants to the vomeronasal organ is actively regulated. In addition, different animals exhibit different stimulant regulation mechanisms. Both volatile and nonvolatile compounds may be perceived, though the perception of nonvolatile compounds requires that the animal make direct contact with the source using its nose or tongue. Lungless salamanders (family Plethodontidae) rely on the vomeronasal organ for habitat selection and mating, using the snout to make deliberate contact with the object being investigated. These animals have a narrow groove close to each nostril that connects the upper lip with the nostril. During nose tapping, fluid moves along the grooves by capillary action and is driven, possibly by ciliary movement, into the extensive vomeronasal organs. In another group of amphibians, the burrowing wormlike caecilians, chemicals are carried to the vomeronasal organs via tentacles. Directly in front of each eye is a small pore leading to a sac that contains a tentacle. The tentacle can be extended through the pore by hydrostatic pressure to make contact with the surrounding soil. A duct connects the tentacular sac with the vomeronasal organ, and it is believed that this is the path along which chemicals are transported. The connection of the vomeronasal organ to the main olfactory epithelium is greatly reduced in these animals.

In snakes and lizards the vomeronasal organ is completely isolated from the nasal cavity. As a consequence, environmental chemicals can enter the organ only via the mouth, and the tongue plays an essential part in chemical transport. In snakes there are no taste buds on the tongue, and chemical transport is probably one of the tongue’s major roles. When snakes and lizards flick their tongues in and out, the tongue moves through a vertical arc. In lizards each extension of the tongue usually involves only one such movement, and the lower surface of the tongue often touches the substrate in front of the lizard. However, the tongues of snakes usually make 3–5 vertical oscillations at each extension, and the tongue usually does not touch the substrate. These movements are rapid, being completed in little more than half a second. (Snakes also make much slower tongue flicks that may serve as warning signals.) It is assumed that, during tongue flicking, odour molecules are trapped in the salivary coating of the tongue, and from there they are transferred to the opening of the vomeronasal organ. Various hypotheses have been put forward to account for the transfer of chemicals from the tongue to the vomeronasal organ, which must occur very quickly; however, the mechanism remains unknown.

In male ungulates, cats, elephants, bats, and some other mammals, access to the vomeronasal organ may be facilitated by curling the lips and exposing the upper teeth, with the nostrils closed. This is called flehmen and is seen during courtship, when it is used by males to assess the estrus state of females, and during the investigation of new odours, when it is used by both males and females to explore their surroundings. Changes in the internal volume of the vomeronasal organ, produced by dilation and compression of blood capillaries, are believed to enhance fluid movement and molecule transport into the lumen. In antelope that exhibit flehmen behaviour, a groove on each side of the hard palate leads to a duct connecting the oral cavity to the vomeronasal organ. Hartebeest and topi, animals that do not exhibit flehmen, lack oral connections to their vomeronasal organs.

The vomeronasal organ is involved in pheromone perception, prey recognition, and habitat selection. Animals such as birds and the great apes do not have vomeronasal organs, and in these animals pheromones are of little or no importance. Even in animals that do possess vomeronasal organs, the olfactory system is involved in pheromone perception. A vomeronasal organ does start to develop in human embryos, and it is present in most, if not all, adults. Its evolutionary development is foreshadowed in fish, in which the vomeronasal gene families are present but are expressed together with olfactory receptor genes in the olfactory epithelium. There is evidence that the nerve pathways from the different receptor types are distinct, though overlapping, in fish.

The significance of the vomeronasal system is that it separates the nervous pathway dealing with innate behavioral and physiological responses from the olfactory pathway that communicates with higher centres of learning and cognition.