Types of neuroglia

Apart from conventional histological and electron-microscopic techniques, immunologic techniques are used to identify different neuroglial cell types. By staining the cells with antibodies that bind to specific protein constituents of different neuroglia, neurologists have been able to discern two (in some opinions, three) main groups of neuroglia: (1) astrocytes, subdivided into fibrous and protoplasmic types; (2) oligodendrocytes, subdivided into interfascicular and perineuronal types; and sometimes (3) microglia.

Fibrous astrocytes are prevalent among myelinated nerve fibres in the white matter of the central nervous system. Organelles seen in the somata of neurons are also seen in astrocytes, but they appear to be much sparser. These cells are characterized by the presence of numerous fibrils in their cytoplasm. The main processes exit the cell in a radial direction (hence the name astrocyte, meaning “star-shaped cell”), forming expansions and end feet at the surfaces of vascular capillaries.

Unlike fibrous astrocytes, protoplasmic astrocytes occur in the gray matter of the central nervous system. They have fewer fibrils within their cytoplasm, and cytoplasmic organelles are sparse, so that the somata are shaped by surrounding neurons and fibres. The processes of protoplasmic astrocytes also make contact with capillaries.

Oligodendrocytes have few cytoplasmic fibrils but a well-developed Golgi apparatus. They can be distinguished from astrocytes by the greater density of both the cytoplasm and the nucleus, the absence of fibrils and of glycogen in the cytoplasm, and large numbers of microtubules in the processes. Interfascicular oligodendrocytes are aligned in rows between the nerve fibres of the white matter of the central nervous system. In gray matter, perineuronal oligodendrocytes are located in close proximity to the somata of neurons. In the peripheral nervous system, neuroglia that are equivalent to oligodendrocytes are called Schwann cells.

Microglial cells are small cells with dark cytoplasm and a dark nucleus. It is uncertain whether they are merely damaged neuroglial cells or occur as a separate group in living tissue.

Neuroglial functions

The term neuroglia means “nerve glue,” and these cells were originally thought to be structural supports for neurons. This is still thought to be plausible, but other functions of the neuroglia are now generally accepted. Oligodendrocytes and Schwann cells produce the myelin sheath around neuronal axons. Some constituent of the axonal surface stimulates Schwann cell proliferation; the type of axon determines whether there is loose or tight myelination of the axon. In tight myelination a glial cell wraps itself like a rolled sheet around a length of axon until the fibre is covered by several layers. Between segments of myelin wrapping are exposed sections called nodes of Ranvier, which are important in the transmission of nerve impulses. Myelinated nerve fibres are found only in vertebrates, leading biologists to conclude that they are an adaptation to transmission over relatively long distances.

Another well-defined role of neuroglial cells is the repair of the central nervous system following injury. Astrocytes divide after injury to the nervous system and occupy the spaces left by injured neurons. The role of oligodendrocytes after injury is unclear, but they may proliferate and form myelin sheaths.

When neurons of the peripheral nervous system are severed, they undergo a process of degeneration followed by regeneration; fibres regenerate in such a way that they return to their original target sites. Schwann cells that remain after nerve degeneration apparently determine the route. This route direction is also performed by astrocytes during development of the central nervous system. In the developing cerebral cortex and cerebellum of primates, astrocytes project long processes to certain locations, and neurons migrate along these processes to arrive at their final locations. Thus, neuronal organization is brought about to some extent by the neuroglia.

Astrocytes are also thought to have high-affinity uptake systems for neurotransmitters such as glutamate and gamma-aminobutyric acid (GABA). This function is important in the modulation of synaptic transmission. Uptake systems tend to terminate neurotransmitter action at the synapses and may also act as storage systems for neurotransmitters when they are needed. For instance, when motor nerves are severed, nerve terminals degenerate and their original sites are occupied by Schwann cells. The synthesis of neurotransmitters by neurons apparently also requires the presence of neuroglial cells in the vicinity.

Finally, the environment surrounding neurons in the brain consists of a network of very narrow extracellular clefts. In 1907 Italian biologist Emilio Lugaro suggested that neuroglial cells exchange substances with the extracellular fluid and in this way exert control on the neuronal environment. It has since been shown that glucose, amino acids, and ions—all of which influence neuronal function—are exchanged between the extracellular space and neuroglial cells. After high levels of neuronal activity, for instance, neuroglial cells can take up and spatially buffer potassium ions and thus maintain normal neuronal function.